ICAR-NRCB - SAIF(Sophiisticated Analytical Instrument Facility)

High-Performance Liquid Chromatography (HPLC)

Instrument details
Make : Waters Corporation, USA
Model & Specification: HPLC with PDA Detector: 515 Binary Pump with 717 plus Autosampler, 2998 PDA Detector and Empower 2 Software
The liquid chromatography separates biochemical compounds based on various physical and/or chemical interactions taking place between the sample/ molecules/ substances and the stationary phase. Because of interactions, the molecules travel according to their relative affinities towards the stationary phase. The component which has more affinity towards the adsorbent travels slower. The component which has less affinity towards the stationary phase travels faster. Since no two components have the same affinity towards the stationary phase, the components are separated. The HPLC is combined with PDA detector.
PDA detector
It is ultraviolet and visible (UV/Vis) spectrophotometer with a photodiode array of 512 photodiodes and an optical resolution of 1.2 nm, the detector operates in the range of 180-800 nm. It is a non-destructive detector.
Quantitative and qualitative analysis.
For Users
1. At present universal C-18 columns are available and if a specific column is required, it may be mentioned.
2. Buffers/solvents required for analysis of biochemicals compounds and operating conditions of the samples be specified.
3. It is mandatory to mention the source and type of biochemicals to be analysed.
4. No toxic chemicals will be accepted and samples must be accompanied by materials safety data sheet.
Charges in Rs.(inclusive of GST)
Test Amount Quantity
Qualitative analysis 3,540/- per sample
Quantitative analysis 7,080/- per sample
Contact Person
Principal Scientist
National Research Centre for Banana
Thogamalai Road, Thayanur Post
Trichy 620102, Tamil Nadu.

Inductively Coupled Plasma – Optical Emission Spectrometer (ICP-OES)

Instrument details
Model : Prodigy 7 (Teledyne Leman Lab)
a) Focal length 500mm
b) Entrance Slit Focal length 60um
c) Image Stabilized Plasma Yes
d) Optical Resolution (pm) 0.007nm @ 200nm
e) 1st measurement from standby 10-15 minutes
f) Need Correction for EIEs No
g) RF Generator Frequency 40.68MHz
h) RF Power 600-2000W
i) Camera CMOS- 3.34MegaPixels
j) Detector L-PAD
k) Pixels Anti-Blooming and Anti-Saturating Yes
l) Spectral Range 165nm – 1100nm
m) Camera Cooling for Reduced Noise -30C
n) Optics Purge 0.7L/min
o) Increase purge for <185nm Not Required
p) Axial/Dual View Tail Cut Air Knife – Zero Maintenance
Working Principle
Inductively Coupled Plasma – Atomic Emission Spectrometry (ICP- AES) is an emission spectrophotometric technique, exploiting the fact that excited electrons emit energy at a given wavelength as they return to ground state after excitation by high temperature Argon Plasma. The fundamental characteristic of this process is that each element emits energy at specific wavelengths peculiar to its atomic character. The energy transfer for electrons when they fall back to ground state is unique to each element as it depends upon the electronic configuration of the orbital. The energy transfer is inversely proportional to the wavelength of electromagnetic radiation,
E = hc/ λ … (where h is Planck’s constant, c the velocity of light and λ is wavelength), and hence the wavelength of light emitted is also unique.
Although each element emits energy at multiple wavelengths, in the ICP-AES technique it is most common to select a single wavelength (or a very few) for a given element. The intensity of the energy emitted at the chosen wavelength is proportional to the amount (concentration) of that element in the sample being analyzed. Thus, by determining which wavelengths are emitted by a sample and by determining their intensities, the analyst can qualitatively and quantitatively find the elements from the given sample relative to a reference standard.
The wavelengths used in AES ranges from the upper part of the vacuum ultraviolet (160 nm) to the limit of visible light (800 nm). As borosilicate glass absorbs light below 310 nm and oxygen in air absorbs light below 200 nm, optical lenses and prisms are generally fabricated from quartz glass and optical paths are evacuated or filled by a non absorbing gas such as Argon.
ICP-OES is used for a wide range of applications in the following industries:
• Environmental
• Oil Analysis
• Pharmaceutical
• Food
• Petrochemical
• Mining
• BioFuels
• Soil samples
• Plant tissue samples
Charges in Rs.(inclusive of GST)
Test Industry University National Lab/ R&Ds Quantity
Standardization & Estimation 1475/- 295/- 708/- Per sample
Estimation for subsequent sample 531/- 118/- 472/- Per sample
Sample preparation 3835/- 885/- 1770/- Per sample
Contact Person
Principal Scientist,
National Research Centre for Banana
Thogamalai Road, Thayanur Post
Trichy 620102, Tamil Nadu.

Gas Chromatography–Mass Spectrometry (GC-MS)

Instrument Detail
Model Agilent Technologies 7890 B GC System / 5977 A MSD (Mass spectral Detector) / Electron Ionization method / NIST Library for identification of compounds
Make Agilent Technologies 7890B GC system/5977A MSD
Software Mass hunter
Column Type Capillary Column HP-5 (Cross bond, 5% Phenyl and 95% dimethylpolysiloxane)
Column length 30m
Column id 250µm
Total GC Run Time 40.50 minutes
GC-MS Principle
The GC-MS instrument consists of two main components. The gas chromatography portion separates different compounds in the sample into pulses of pure chemicals based on their volatility by flowing an inert gas (Mobile phase), which carries the sample, through a stationary phase fixed in the column. Spectra of compounds are collected as they exit a chromatographic column by the mass spectrometer, which identifies and quantifies the chemicals according their mass-to-charge ratio (m/z). These spectra can then be stored on the computer and analysed.
MS conditions
Mass Range 20-550amu
Mode Full Scan Mode
Type of Ionization Electron Ionization (EI)
Electron energy 70ev
Transfer line and source temperature 230ºC
Library NIST 2014
This facility can be used for the identification of semi-volatile and volatile organic compounds (minimum sample size required is 2 µl).
User Instruction
The samples should be prepared by standard extractionand clean up procedure in consultation with National Reference Laboratory.
Charges in Rs.(inclusive of GST)
Test Amount Quantity
GCMS Analysis and preparation of report 3,540/- Per sample
Contact Person
Senior Scientist,
National Research Centre for Banana
Thogamalai Road, Thayanur Post
Trichy 620102, Tamil Nadu.

Gamma Chamber 2000/ BI 2000

Instrument Detail
Model BI2000 with Co60 as source with its maximum capacity 30 TBq (810 Ci)
Make BRIT, Mumbai
Co60 source in the BI 2000/GC 2000 will emit gamma rays that have the potential to mutate any living cells or organs. The variability caused through mutagenesis can be exploited in the improvement programmes. The gamma rays have the potential to extend the shelf life of perishable commodities.
Gamma Irradiation Chamber is versatile equipment used for research studies in fields such as:
• Mutation Breeding
• Irradiation of Seeds, Bulbs, Tubers and Scions
• Biological and Genetic Effects of Radiations
• Sterile Male Insect Technique
• Sterile Pollen technique
• Post harvest shelf life studies
Gamma Irradiation Chamber can also be used in many other research applications which require irradiation of materials with ionizing radiations at varying doses.
Testing in Gamma Chamber : One dose Rate
Radiation Intensity (Gy) Govt.Institutions / colleges / Students Private / Corporate clients
0 to 100 Rs.100.00 + GST Rs.500.00 + GST
100 to 500 Rs.200.00 + GST Rs.1000.00 + GST
500 to 1000 Rs.500.00 + GST Rs.1500.00 + GST
> 1000 Rs.1000.00 + GST Rs.2000.00 + GST
Contact Person
Principal Scientist,
National Research Centre for Banana
Thogamalai Road, Thayanur Post
Trichy 620102, Tamil Nadu.


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