A 10-days training program on “Cutting-Edge Techniques in Banana Tissue Culture and Its Application” was successfully conducted at the ICAR-National Research Centre for Banana (ICAR-NRCB), Tiruchirappalli, from 11th to 20th December 2024. The program aimed to enhance participants' proficiency in advanced tissue culture techniques, ensure the production of high-quality, genetically uniform plants, and promote the application of somatic embryogenesis in banana improvement. The Course Directors of this training were Dr. S. Backiyarani, Principal Scientist, and Dr. R. Selvarajan, Director, ICAR-NRCB. The course coordinators wereDr. M. S. Saraswathi, Principal Scientist, Dr. C. Anuradha, Senior Scientist and Dr. K. Nagendran, Scientist. The training program was formally inaugurated by Dr. R. Selvarajan, Director, ICAR-NRCB, in his inaugural address, emphasized the critical role of tissue culture in modern agriculture, utility of ECS in genetic manipulations like transforming and editing are of prime importance and highlighted ICAR-NRCB’s contributions to research and capacity building in this field. A total of nine participants, including researchers, academicians, and industry professionals, attended the program. The training featured a combination of lectures and practical sessions conducted by experienced scientists from ICAR-NRCB and other reputed institutions. Topics covered included somatic embryogenesis techniques for producing high-quality planting material, virus indexing, advanced biotechnological techniques for crop improvement. Key aspects of the training focused on direct and zygotic regeneration, embryogenic cell multiplication, and high-throughput production using SERV bioreactors. The program also addressed critical biotechnology themes such as genetic fidelity, virus indexing, transgenics for stress tolerance, CRISPR/Cas genome editing, and mutation breeding. Additionally, metabolomics approaches for optimizing secondary metabolite production and advanced protocols for genetic transformation and guide RNA design were explored. Hands on training has been provided to all the participants especially on all the stages of somatic embryogenesis. The program concluded with a valedictory session, during which participants shared feedback and expressed appreciation for the well-structured training. Certificates of participation were awarded by the Director, who emphasized the importance of bioreactor technology in banana tissue culture and highlighted ICAR-NRCB’s 15 years of dedication to developing these technologies. The training program received overwhelmingly positive feedback for its comprehensive curriculum, expert faculty, and hands-on learning opportunities. Participants expressed confidence in applying the techniques in their professional and research endeavors. Overall, the program successfully achieved its objectives of capacity building and knowledge dissemination, further strengthening the role of tissue culture techniques in banana improvement and industry applications.