Our Facility is equipped with sophisticated analytical equipment for meeting the needs of Scientists as well as farmers.
High-performance liquid chromatography
Inductively Coupled Plasma – Optical Emission Spectrometer
Gas Chromatography–Mass Spectrometry
Gamma Chamber 2000/ BI 2000
Explore our advanced analytical equipment and facilities designed to meet your research and analytical needs. Our equipment and facilities are trusted tools for researchers, scientists, and industry professionals seeking precise analysis and innovative solutions.
| Make | M/s. Waters Corporation, USA |
| Model | 515 binary pump, 717 plus autosampler, C18 (universal) Column and 2998 PDA detector |
| Specifications | Phytochemicals analyses |
Liquid chromatography is a separation technique in which the components (biochemical / analytes) to be separated are distributed between two phases, one of which is stationary phase while the other (the mobile phase) is a liquid that moves in a definite direction. Different components of a sample are transported in the mobile phase through a stationary phase. Analytes usually have differential interactions with the mobile and stationary phases. Since the stationary phase is the fixed one, then those solutes, which have stronger interactions with the stationary phase will tend to move slower than others, which have lower or no interactions with the stationary phase will tend to move faster. Therefore, chromatographic separations depend on the strength of the interactions of the analytes with the phases. The time required for an analyte to travel through the column after injection till the analyte reaches the detector is termed the retention time (Rt).
The liquid chromatography separates biochemical compounds based on various physical and/or chemical interactions taking place between the sample/ molecules/ substances and the stationary phase...
Plant samples, Food samples and Microbial samples.
Quantitative and qualitative analysis.
| Nature of samples | ICAR-NRCB | Govt.R&D Lab.including Colleges | Private |
|---|---|---|---|
| Liquid samples ready to inject in the HPLC | Rs.800/- | Rs.1000/- | Rs.1500/- |
| Samples requiring extraction and processing | Rs.1000/- | Rs.1200/- | Rs.1800/- |
Model : Prodigy 7 (Teledyne Leman Lab)
| a) Focal length | 500mm |
| b) Entrance Slit Focal length | 60um |
| c) Image Stabilized Plasma | Yes |
| d) Optical Resolution (pm) | 0.007nm @ 200nm |
| e) 1st measurement from standby | 10-15 minutes |
| f) Need Correction for EIEs | No |
| g) RF Generator Frequency | 40.68MHz |
| h) RF Power | 600-2000W |
| i) Camera | CMOS- 3.34MegaPixels |
| j) Detector | L-PAD |
| k) Pixels Anti-Blooming and Anti-Saturating | Yes |
| l) Spectral Range | 165nm – 1100nm |
| m) Camera Cooling for Reduced Noise | -30C |
| n) Optics Purge | 0.7L/min |
| o) Increase purge for <185nm | Not Required |
| p) Axial/Dual View Tail Cut | Air Knife – Zero Maintenance |
Inductively Coupled Plasma – Atomic Emission Spectrometry (ICP- AES) is an emission spectrophotometric technique, exploiting the fact that excited electrons emit energy at a given wavelength as they return to ground state after excitation by high temperature Argon Plasma. The fundamental characteristic of this process is that each element emits energy at specific wavelengths peculiar to its atomic character. The energy transfer for electrons when they fall back to ground state is unique to each element as it depends upon the electronic configuration of the orbital. The energy transfer is inversely proportional to the wavelength of electromagnetic radiation,
E = hc/ λ … (where h is Planck’s constant, c the velocity of light and λ is wavelength), and hence the wavelength of light emitted is also unique.
Although each element emits energy at multiple wavelengths, in the ICP-AES technique it is most common to select a single wavelength (or a very few) for a given element. The intensity of the energy emitted at the chosen wavelength is proportional to the amount (concentration) of that element in the sample being analyzed. Thus, by determining which wavelengths are emitted by a sample and by determining their intensities, the analyst can qualitatively and quantitatively find the elements from the given sample relative to a reference standard.
The wavelengths used in AES ranges from the upper part of the vacuum ultraviolet (160 nm) to the limit of visible light (800 nm). As borosilicate glass absorbs light below 310 nm and oxygen in air absorbs light below 200 nm, optical lenses and prisms are generally fabricated from quartz glass and optical paths are evacuated or filled by a non absorbing gas such as Argon.
ICP-OES is used for a wide range of applications in the following industries:
| Test | Industry | University | National Lab/ R&Ds | Quantity |
| Standardization & Estimation | 1475/- | 295/- | 708/- | Per sample |
| Estimation for subsequent sample | 531/- | 118/- | 472/- | Per sample |
| Sample preparation | 3835/- | 885/- | 1770/- | Per sample |
| Model | Agilent Technologies 7890 B GC System / 5977 A MSD (Mass spectral Detector) / Electron Ionization method / NIST Library for identification of compounds |
| Make | Agilent Technologies 7890B GC system/5977A MSD |
| Software | Mass hunter |
| Column Type | Capillary Column HP-5 (Cross bond, 5% Phenyl and 95% dimethylpolysiloxane) |
| Column length | 30m |
| Column ID | 250µm |
| Total GC Run Time | 40.50 minutes |
The GC-MS instrument consists of two main components. The gas chromatography portion separates different compounds in the sample into pulses of pure chemicals based on their volatility by flowing an inert gas (Mobile phase), which carries the sample, through a stationary phase fixed in the column...
| Mass Range | 20-550amu |
| Mode | Full Scan Mode |
| Type of Ionization | Electron Ionization (EI) |
| Electron energy | 70eV |
| Transfer line and source temperature | 230ºC |
| Library | NIST 2014 |
This facility can be used for the identification of semi-volatile and volatile organic compounds (minimum sample size required is 2 µl)...
The samples should be prepared by standard extraction and clean-up procedure in consultation with the National Reference Laboratory.
| Test | Amount | Quantity |
| GCMS Analysis and preparation of report | 3,540/- | Per sample |
| Model | BI2000 with Co60 as source with its maximum capacity 30 TBq (810 Ci) |
| Make | BRIT, Mumbai |
Co60 source in the BI 2000/GC 2000 will emit gamma rays that have the potential to mutate any living cells or organs. The variability caused through mutagenesis can be exploited in improvement programs. The gamma rays have the potential to extend the shelf life of perishable commodities.
Gamma Irradiation Chamber is versatile equipment used for research studies in fields such as:
Gamma Irradiation Chamber can also be used in many other research applications which require irradiation of materials with ionizing radiations at varying doses.
| Radiation Intensity (Gy) | Govt. Institutions / Colleges / Students | Private / Corporate Clients |
|---|---|---|
| 0 to 100 | Rs.100.00 + GST | Rs.500.00 + GST |
| 100 to 500 | Rs.200.00 + GST | Rs.1000.00 + GST |
| 500 to 1000 | Rs.500.00 + GST | Rs.1500.00 + GST |
| > 1000 | Rs.1000.00 + GST | Rs.2000.00 + GST |